Fig. 1

Pyroptosis occurs in microglia following spinal cord injury. A pyroptosis-related gene expression patterns at days 1, 3, 7, and 28 after SCI; B Representative immunoblots showing the level of GSDMD protein at Days 1, 3, 7, 14, and 28 following SCI. measurement of the relative GSDMD protein levels (n = 3); C Immunofluorescence of the spinal cord 7 days after injury reveals that GSDMD is mostly expressed in microglia (IBA1) but less in neurons (NeuN) or astrocytes (GFAP); D Quantification of fluorescence intensity of GSDMD at Day 7 postinjury(n = 3); E Flow cytometry demonstrating that Treg cell infiltration is significantly increased in the spinal cord 7 days after injury(n = 3)