Fig. 5

Exosomes derived from Treg cell promote motor function recovery and supress microglia pyroptosis after SCI in vivo. A The experimental scheme for exosomes injection after SCI; B BMS was used to functionally grade mice in different groups up to 28 days post-injury. C Footprint analysis at Day 28 postinjury demonstrate better functional recovery in SCI + Exosomes mice; D The footprints quantifcation of mice walking 28 days after SCI(n = 6); E Swimming test at Day 28 postinjury demonstrate better functional recovery in SCI + Exosomes mice; F Louisville Swim Scale score in SCI + PBS and SCI + Exosomes mice at Day 28 postinjury(n = 6); GMEP analysis was used as electrophysiological assessment in SCI + PBS and SCI + Exosomes mice at Day 28 postinjury; H Quantification of peak-to-peak MEP amplitudes and latencies in SCI + PBS and SCI + Exosomes mice (n = 6); I Representative immunofluorescence images for GSDMD(pyroptosis) and IBA1(microglia) expression in SCI + PBS and SCI + Exosomes mice at Day 7 postinjury; J Quantification of fluorescence intensity at Day 7 postinjury (n = 3); K Representative immunoblots showing levels of pyroptosis-related protein in injured spinal cord at days 7 postinjury in SCI + PBS and SCI + Exosomes mice