Fig. 3
Ep167-193 from PA4554 is a novel predominant epitope that shows strong immunogenicity in mice. A. Heatmap of ten peptides showing their reactivity to sera from 100 patients who had recovered from PA infection. B. Summary of the immunoreactivity results of each peptide. The bar represents the median fluorescence intensity (MFI) of each peptide as determined by the Luminex assay. Multiple comparisons among the groups were analyzed by one-way ANOVA. *P < 0.05. C. The bars represent the titers of anti-peptide antibodies in sera from mice immunized with the corresponding peptide conjugated to the KLH carrier protein (n = 10). The top two most immunogenic peptides, PA2398 Q737-K754 and PA4554 C167-W193, are labeled with a blue background. Multiple comparisons among the groups were analyzed by one-way ANOVA. *P < 0.05