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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Extracellular vesicles from IFN-γ-primed mesenchymal stem cells repress atopic dermatitis in mice

Fig. 1

Characterization of IFN-γ-iMSCs and IFN-γ-iMSC-EVs. A Flow cytometry analysis of IFN-γ-iMSCs. The reactivities of IFN-γ-iMSCs against positive (CD90, CD105, and CD73) or negative (CD45 and CD34) markers of MSCs were tested. The IgG isotype was used as a non-specific control (black peaks). B qPCR analysis of IDO1 mRNA in iMSCs and IFN-γ-iMSCs. n = 3. Data are presented as the mean ± SE. *p < 0.05. C Morphology of IFN-γ-iMSC-EVs under cryo-TEM. Scale bar = 200 nm. D Size distribution of IFN-γ-iMSC-EVs shown by NTA and DLS. The surface charge and polydispersity index (PDI) was also measured by DLS. E Western blot analyses for markers of extracellular vesicles (CD63, CD81, and TSG101) or cellular organelles (GM130 and calnexin) in IFN-γ-iMSCs and IFN-γ-iMSC-EVs. F Expression analysis of EV markers (CD9, CD63, and CD81) in IFN-γ-iMSC-EVs by flow cytometry. G Biodistribution of IFN-γ-iMSC-EVs. DNCB-induced AD mice were subcutaneously injected with DiR only or DiR-labeled IFN-γ-iMSC-EVs. After 8 h, the localization of DiR or DiR-labeled IFN-γ-iMSC-EVs in whole body and major internal organs was observed by in vivo imaging

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