Fig. 3

Blockade of AD progression by IFN-γ-iMSC-EVs. AD was induced by DNCB in NC/Nga mice. AD mice were subcutaneously administered with PBS or IFN-γ-iMSC-EVs (50 or 500 μg) for negative control and test groups, respectively. A Gross appearance of the skin lesions of AD animals that received IFN-γ-iMSC-EVs. B Analysis of dermatitis severity score. n = 5. Data are presented as mean ± SE. ***p < 0.001; ^#p < 0.05. C Immunoblot analysis of IL-4Rα and IL-13Rα1 in skin tissues from AD mice that received IFN-γ-iMSC-EVs. n = 5. Data are presented as mean ± SE. *p < 0.05; ^##p < 0.01. D Immunoblot analysis of phosphorylated JAK1 in skin tissues from AD mice that received IFN-γ-iMSC-EVs. The density of phosphorylated JAK1 was normalized to that of total JAK1. n = 5. Data are presented as the mean ± SE. *p < 0.05; ^#p < 0.05. E Immunoblot analysis of phosphorylated STAT6 in skin tissues from AD mice that received IFN-γ-iMSC-EVs. The density of phosphorylated STAT6 was normalized to that of total STAT6. n = 5. Data are presented as mean ± SE. **p < 0.01; ^##p < 0.01