Fig. 2
From: Splenic-targeting biomimetic nanovaccine for elevating protective immunity against virus infection
MR-PLGA/OVA NVs mediated targeted delivery of antigen to BMDCs in vitro. A CLSM images of BMDCs following incubation with free OVA, or MR-PLGA/OVA NVs for 12 h. BMDCs were pretreated with mannose for 2 h to block mannose receptor. The scale bar represents 10 μm. B Uptake level of MR-PLGA/OVA NVs with or without mannose pretreatment (n = 3). C Uptake of free OVA and MR-PLGA/OVA NVs in BMDCs as assessed by flow cytometry. The BMDCs were pretreated as described in (A). (I: free FITC-OVA; II: MR-PLGA/FITC-OVA NVs (w/ Man); III: MR-PLGA/FITC-OVA NVs.)