Fig. 5

Biocompatibility of FDM 3D-printed polymer discs made of PLA, PMMA and PC was investigated using SaOS-2 osteoblasts and hMSC. A The viability of SaOS-2 osteoblasts and hMSC was assessed by MTT assay at 24 and 48 h and compared with the untreated sample (standard 24-well tissue culture plate). Statistical significance was analyzed by Two-way ANOVA and Dunnett's post hoc test compared with the untreated sample (ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001). B Representative microscopic images of FDM printed polymer discs cultivated with SaOS-2 osteoblasts for 24 and 48 h. Images were taken after staining with MTT. Scale bar measures 500 µm. Images were acquired using an Olympus CKX41 (Olympus, Japan) microscope equipped with an Olympus XM10 camera (Olympus, Japan) and associated cellSens Standard software (version 1.9 build 11,514, Olympus, Japan)