Fig. 4

Gene expression and hematology analyses. a–f Expression levels of iron-containing proteins in the liver during 1 month after the nanoparticle administration: transferrin, DMT1, iron responsive element of DMT1 (DMT1-IRE), ferroportin, light and heavy chains of ferritin (Ferritin-L and Ferritin-H, respectively). g–r Hematological analysis within 2 months after the nanoparticle injection: red blood cell count (RBC), hematocrit (HCT), hemoglobin concentration (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), standard deviation of red cell distribution width (RDW_SD), coefficient of variation of red cell distribution width (RDW_CV), white blood cell count (WBC), platelet count (PLT), plateletcrit (PCT), platelet-large cell ratio (P_LCR). Asterisks indicate significant difference from the control group (violet bar in each graph): Welch’s t-test, *p < 0.05; **p < 0.01; ***p < 0.001