Table 1 List of gene-specific primers for real-time PCR
From: Influence of magnetic nanoparticle biotransformation on contrasting efficiency and iron metabolism
|  | Protein | Forward (5′ → 3′) | Reverse (5′ → 3′) | PCR (bp) |
|---|---|---|---|---|
1 | DMT1 | TCCCACATTCCACTGGAGACT | AGAGCAGCTTAAATCCAGCCA | 210 |
2 | DMT1 (IRE only) | CGTCTGCTCCATCAACATGTACT | GTATCTTCGCTCAGCAGGACTTTC | 207 |
3 | Ferritin heavy chain | GAAGCTGCAGAACCAGCGAG | CCTGTTCACTCAGATAATACGTCT | 215 |
4 | Ferritin light chain | ATCTGCATGCCCTGGGTTC | GAGTGAGGCGCTCAAAGAGA | 201 |
5 | Ferroportin | CCGAGATGGATGGGTCTCCT | GCCACATTTTCGACGTAGCC | 220 |
6 | Transferrin | GCAGTGTCAGAGCACGAGAA | GGCTTCAGGTTGTTCGGAGT | 209 |
7 | GAPDH | TCATGACCACAGTCCATGCC | ATCCACGACGGACACATTGG | 212 |