Fig. 6
Degradation of MNPs in Kupffer cells from 7 days to 15 months post-administration of the MNPs with different coatings. Immunohistochemical analysis of MNP accumulation in Kupffer liver cells (labeled with the macrophage F4/80 antibody) and intracellular iron visualized by Prussian blue staining and a neutral red counterstain. In the lower right corner of each panel (dashed box) an amplified image of the Kupffer cells is shown. Representative images of 15 sections per condition are shown (n = 7). Scale bar 40 μm (20X), 63X or 100X (bottom right inset) zoom objective. In the images, the area where the zoom shown in the bottom right inset has been made has been marked with a black arrow. In the figures that the area in question is not indicated, it is because it coincides with the bottom right inset area where the image is placed or the zoom corresponds to another of the images