Fig. 5
From: Acidic and hypoxic tumor microenvironment regulation by CaO2-loaded polydopamine nanoparticles
Anti-metastatic and anti-angiogenesis effect. A Diagrammatic representation of the therapeutic experiment (n = 5, mean ± SD). Tumor inoculation and intravenous injections were performed as described. B Number of lung metastases tumor nodules from different treatment groups. C Representative digital images showing the formation of tumor nodules in the lungs. Arrowheads indicate macroscopically detected pulmonary metastasis foci. D H&E staining of lung sections from different treatment groups. Scale bar: 200 μm. E Immunohistochemistry staining displayed expression of CD31 and VEGF in tumor tissues after different treatments. (Brown: antibody, Blue: cell nucleus, Scale bar: 200 μm). F Numbers of blood vessels/field and G percentages of VEGF stained area were analyzed in tumor sections. Results are the mean ± SD of 5 experiments. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001