Table 1 The primer sequences used for qRT–PCR
Gene | Forward primer | Reverse primer |
|---|---|---|
GAPDH | CTCAACTACATGGTCTACATGTTCCA | CTTCCCATTCTCAGCCTTGACT |
Acan | TCCACATCAGAAGAGCCATAC | AGTCAAGGTCGCCAGAGG |
Col2a1 | CTTAGGACAGAGAGAGAAGG | ACTCTGGGTGGCAGAGTTTC |
SOX9 | CAGTCCCAGCGAACGCACAT | TGCTGCTGCTGCTCGCTGTA |
MMP3 | GGCTGTGTGCTCATCCTACC | TGGAAAGGTACTGAAGCCACC |
MMP13 | AAAGAACATGGTGACTTCTACC | ACTGGATTCCTTGAACGTC |
HIF-2α | CTGAGGAAGGAGAAATCCCGT | TGTGTCCGAAGGAAGCTGATG |
IL-6 | AGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
Adamts-5 | TGTGGTGCGCCAAGGCCAAA | CCCTGTGCAGTAGCGGCCAC |
COX-2 | GATGACGAGCGACTGTTCCA | CAATGTTGAAGGTGTCCGGC |