Fig. 2
a The Perls Prussian stained SCC-9 cells obtained after incubation with FeIIITA@HA nanoprobes with different Fe concentrations with or without CD44 Ab. b Cell viabilities of SCC-9 cells treated with the FeIIITA@HA nanoprobes with different Fe concentrations under different conditions. c Fluorescence images of SCC-9 cells treated with different agents, respectively, followed by DAPI staining for showing the cell nuclei and DCFH-DA staining for showing the intracellular ROS. d The intracellular GSH contents of SCC-9 cells after co-incubation with nanoprobes with different Fe concentrations with or without CD44 Ab. e The intracellular GPX4 levels of SCC-9 cells under different conditions. f Cell images acquired after Live-Dead staining for showing the thermal ablation of FeIIITA@HA nanoprobes under 650 nm laser irradiation (1.0 W·cm−2) for 10 min. Note: the embedded scale bars in the cell images in frame a, c and f correspond to 50 μm, 20 and 50 μm, respectively. Data in frame b, d and e plotted as mean ± standard deviation, n = 3. Statistical significance was determined by one-way ANOVA with a Tukey’s post hoc test (***p < 0.001; **p < 0.01; *p < 0.05)