Fig. 4

a SEM of IO@FuDex³. b IVIS images of the growth of luciferase-expressing 4T1 tumors at 4 weeks after first treatment with IO@FuDex³ formulations. c, d Tumor volumes and survival curves of mice receiving treatment with different IO@FuDex³ formulations. e, f Significant increase in CD8⁺ and CD4⁺ T cells after treatment with IO@FuDex³ formulations. Data in c, d are expressed as mean ± SD, n = 8 biologically independent animals. ^#P < 0.05 and ^##P < 0.01 compared with the control group (IgG); *P < 0.05 and **P < 0.01 compared between groups using a paired two-way Student’s t-test [85].