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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Lactate oxidase/catalase-displaying nanoparticles efficiently consume lactate in the tumor microenvironment to effectively suppress tumor growth

Fig. 4

Lactate consumption and cell viability of CT26 cells under normoxic conditions after AaLS-ST, AaLS/LOX, or AaLS/LOX/CAT treatments. The lactate concentration and cell viability profiles of CT26 cells in the media treated with AaLS-ST (a), AaLS/LOX (b), or AaLS/LOX/CAT (c) at various sample concentrations. d Fluorescence microscopic images showing CT26 cells treated with AaLS-ST, AaLS/LOX, or AaLS/LOX/CAT, followed by incubation with 2 μM CM-H2DCFDA in the dark for 30 min at 37 °C (scale bar, 100 μm). e Flow cytometric results showing annexin V/7-AAD-stained CT26 cells upon treatment with AaLS-ST, AaLS/LOX, or AaLS/LOX/CAT. All cells were analyzed after 6 or 12 h of sample treatments. In each plot, the upper-right quadrant indicates late apoptosis and necrosis, the lower-right quadrant indicates early apoptosis, and the lower-left quadrant indicates the normal state of cells. The relative populations of each quadrant were individually marked. Real-time live imaging of CT26 cells treated with AaLS-ST (f), AaLS/LOX (g), and AaLS/LOX/CAT (h) are also shown. The nuclei of cells were stained with Hoechst 33342 and visualized in blue (scale bar, 10 μm)

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