Fig. 3

The NAMPT level was lower in isolated ADMSC^old-EVs than in ADMSC^young-EVs. a Schematic diagram of the isolation of ADMSC^young-EVs and ADMSC^old-EVs. b Morphological analysis of ADMSC^young-EVs and ADMSC^old-EVs by TEM. Scale bar = 200 nm. c Size distribution and zeta potential of ADMSC^young-EVs and ADMSC^old-EVs, n = 6. d Relative amount of secreted EVs in ADMSC^young and ADMSC^old, n = 6. e Immunofluorescence staining of Alix (green) and CD9 (red) in ADMSC^young-EVs and ADMSC^old-EVs. Scale bar = 50 μm. f Western blot analysis and quantification of the expression of CD9, CD63, and Alix proteins as well as NAMPT in ADMSC^young-EVs and ADMSC^old-EVs, n = 3. Data are presented as the mean ± SD (ns: no significance; **: P < 0.01; ***: P < 0.001)