Fig. 7

Penetration and synergistic therapy. a Illustration of the tumor penetration ability of the nanoplatform. b1 CLSM images of the nanoplatform permeating the tumors in MDA-MB-231 tumor-bearing mice and b2 the corresponding 3D reconstruction surface plot after intravenous injection of THGP or HGP. c Schematic illustration of MDA-MB-231 cell tumor xenograft establishment, PBS and various nanoplatfrom administration modalities, and analysis. d Body weight curves of mice in various groups during the treatment process (n = 5). e Relative tumor volumes of mice in the various groups during the treatment (n = 5). f Representative digital images of MDA-MB-231 tumor-bearing mice after various treatments. g Representative digital images of excised tumors after various treatments and h the corresponding tumor weight curves (n = 5). i Immunofluorescence images of mouse tumors stained for HIF-1α (red, hypoxia level) and with DAPI (blue, nuclei) after various treatments (scale bar: 200 µm). j H&E, TUNEL, and PCNA staining of the tumor tissues of various treatment groups (scale bar: 200 µm)