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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Endothelial cell-derived exosomes boost and maintain repair-related phenotypes of Schwann cells via miR199-5p to promote nerve regeneration

Fig. 3

MiR199-5p was a key EC-EXO cargo in promoting SC repair-related phenotypes. The miRNAs in SCs treated with EC-EXO were profiled by the miRNA sequencing analysis. A Different miRNAs were detected in EXO-treated and control groups on a co-expression Venn diagram. B Gene Ontology (GO) term analyses of significantly changed miRNAs in control group and EXO-treated group regarding their involvement in biological processes (BP), cellular components (CC) and molecular functions (MF). C Significantly enriched KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways related to the differentially expressed miRNAs obtained from the comparison of control group and EXO-treated group. D The heat map displayed the expression ratios of different microRNAs between control group and EXO-treated group. E Volcano plots showed the up-regulated microRNAs (red), down-regulated microRNAs (green) and non-changing microRNAs (gray) between EXO-treated SCs and control SCs. F Real-time qRT-PCR validated novel-miR199-5p expression in EXO-treated SCs. Data are expressed as mean ± SD (n = 3). G Quantitative PCR analysis of novel-miR199-5p expression levels in SCs transfected with mimic, mimic negative control (Mi-NC), inhibitor and inhibitor negative control (In-NC). Data are expressed as mean ± SD (n = 3). H Cell growth curve was detected by CCK-8 assay in different groups. The data are expressed as mean ± SD (n = 3). I Representative EdU staining images in different groups. Scale bar, 100 μm. J Statistical evaluation of percentage of EdU-positive SCs. The data are expressed as mean ± SD (n = 3). K Representative images of the colony formation in different groups. Scale bar, 500 μm. L Statistical results of the colony formation in each group. The data are expressed as mean ± SD (n = 3). M Representative images of vertical migration of SCs in different groups for 24 h. Scale bar, 100 μm. N The number of migrated SCs was counted and analyzed. The data are expressed as mean ± SD (n = 3). ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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