Fig. 7

Histological changes in injured sciatic nerve with the treatment of EC-EXO. A Representative images of HE and Masson staining sciatic nerve longitudinal and cross sections in sham, PNI and EXO groups 28 days following the treatment of EC-EXO (n = 3). Scale bar, 20 μm. B Representative images of toluidine blue (TB) staining sciatic nerve in each group (n = 3). Scale bar, 20 μm. C Representative TEM images of sciatic nerve in each group (n = 3). Scale bar, 10, 2 and 1 μm. D Statistical analysis of the number of axons with myelin. The data are expressed as mean ± SD (n = 3). E Statistical analysis of the thickness of myelin sheath. The data are expressed as mean ± SD (n = 3). F Statistical analysis of G-ratio; The data are expressed as mean ± SD (n = 3). ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001