Fig. 6

Apoptosis (Annexin V) and cell viability (MTT) analysis. a Apoptosis analysis using a fluorescence-activated cell sorting (FACS) assay after treatment with CNT, DOX, CNT-DOX, and CNT-DOX with GEN (caveolin endocytosis inhibitor) for 24 h. As a positive control, hydrogen peroxide (H2O2) was used. Data shows significant apoptosis on CNT-DOX compared with DOX. b Bar graphs illustrating apoptosis levels quantified by Annexin in H69AR cells after nanodrug treatment. c Cell viability analysis using an MTT assay after treatment with CNT, DOX, CNT-DOX, Caveolin inhibitor (GEN), and CNT-DOX with GEN for 48. Data shows significant decrease of cell population on CNT-DOX but recovered after treatment of caveolin inhibitor. All data represent the mean ± SEM (n = 10). ***p < 0.001