Fig. 4

ThT assay of Aβ aggregation. The inhibitory effects of anti-amyloid β (Aβ)O antibody 6H4, anti-Aβ antibody 3D6, and anti-Aβ aggregate-specific antibody GTX134510 on Aβ42 aggregation were evaluated using a thioflavin T (ThT) assay. A Aβ alone (white circles) continued to increase the self-aggregation rate even after 1 h of incubation with ThT. In contrast, 6H4 (black circles) showed stable inhibition of Aβ42 aggregation throughout the incubation period. However, compared with 6H4, GTX 134,510 (black triangles) showed a significantly decreased inhibition effect for approximately 3 h of incubation; 3D6 (white squares) showed a significantly decreased inhibition effect after approximately 8 h of incubation. B Compared with Aβ alone (white bar) and GTX134510 (hatched bar), 6H4 (black bar) showed a significant inhibition of the Aβ42 aggregation rate after 6 h of incubation. C Compared with Aβ alone, 6H4 maintained a 99% inhibition rate on Aβ42 aggregation even after 12 h of incubation; this was significantly higher than the inhibition rate maintained by 3D6 (horizontal striped bar). Values are expressed as mean ± standard error of the mean. Statistical evaluations were performed using one-way or two-way analysis of variance with a Tukey’s post-hoc test. **p < 0.01