Fig. 6

In vitro antitumor effect. A Fluorescence images of cell proliferation after various treatments evaluated by EdU assay. Red fluorescence represented proliferative cell, and blue fluorescence represented cell nuclei counterstained by Hoechst. Scale bar: 100 μm. B Cell apoptosis by FCM after different treatments. C Invasion cells assessed by Transwell assay. Scale bar: 100 μm. D The percentage of positive cell rate examined from A by Image J. E Quantification of cell apoptosis from B. F Quantification of the invading cells from C. Data are presented as mean ± SD (n = 3, *p < 0.05, **p < 0.01)