Fig. 2
In vitro anti-colorectal cancer effect of HCR NPs. A–D The viability of DLD-1 and HCT116 cells following treatment with HA-HCQ, IR820, CLT, CLT + IR820, CLT-IR820, HCR NPs, IR820 + Laser, CLT + IR820 + Laser, CLT-IR820 + Laser, and HCR NPs + Laser (λ = 808 nm, P = 1 W/cm2; 40 s) at different concentrations. E The proliferation of DLD-1 and HCT116 cells treated with CLT, CLT + IR820 + Laser, CLT-IR820 + Laser, and HCR NPs + Laser measured by the EdU assay. Scale bar: 50 μm. (λ = 808 nm, P = 1 W/cm2; 40 s). F, G LDH assay of DLD-1 and HCT116 cells cocultured with CLT, CLT + IR820 + Laser, CLT-IR820 + Laser, and HCR NPs + Laser. (λ = 808 nm, P = 1 W/cm2; 40 s). ***P < 0.001