Fig. 3
HCR NPs induce apoptosis in colorectal cancer cells. A Apoptosis of CLT, CLT + IR820 + Laser, CLT-IR820 + Laser, and HCR NPs + Laser was evaluated by Annexin V-FITC/PI staining in DLD-1 and HCT116 cells. Quantification of the apoptotic cell ratio is shown. (λ = 808 nm, P = 1 W/cm2; 2 min). B, C Immunoblot analysis of apoptotic markers in DLD-1 and HCT116 cells treated with CLT, CLT + IR820 + Laser, CLT-IR820 + Laser, or HCR NPs + Laser. (λ = 808 nm, P = 1 W/cm2; 2 min)