Fig. 4
HCR NPs-mediated ROS production promotes colorectal cancer cell death. A, B Flow cytometry analysis of intracellular ROS generation in DLD-1 and HCT116 cells treated with CLT, CLT + IR820 + Laser, CLT-IR820 + Laser, or HCR NPs + Laser using DCFH-DA as a probe. (λ = 808 nm, P = 1 W/cm2; 2 min). C Immunoblot analysis of PRDX2 in DLD-1 and HCT116 cells treated with CLT, CLT + IR820 + Laser, CLT-IR820 + Laser, and HCR NPs + Laser. (λ = 808 nm, P = 1.0 W/cm2; 2 min). D Fluorescent images of DCFH-DA-stained DLD-1 cells and HCT116 cells treated with HCR NPs + Laser in combination with or without NAC treatment. Scale bar: 50 μm. (λ = 808 nm, P = 1.0 W/cm2; 2 min). E, F Flow cytometry analysis of intracellular ROS generation in HCR NPs + Laser -treated DLD-1 and HCT116 cells with or without NAC treatment using DCFH-DA as a probe. (λ = 808 nm, P = 1 W/cm2; 2 min). G, H Cell viability of DLD-1 cells and HCT116 cells treated with HCR NPs + Laser in combination with or without NAC treatment. (λ = 808 nm, P = 1.0 W/cm2; 40 s). ***P < 0.001. I Immunoblot analysis of apoptotic marker in DLD-1 and HCT116 cells treated with HCR NPs + Laser in combination with or without NAC treatment. (λ = 808 nm, P = 1.0 W/cm2; 2 min). ***P < 0.001