Fig. 1
Extraction and purification of LPS, IpaB44-310 and IpaC. a Silver staining of LPS extracted from Sd1 (lane 1) and Sf2a (lane 2). b–d Characterization of IpaB44-310—b SDS PAGE of expressed IpaB44-310. Lanes represent bacterial cell lysates of uninduced control and IPTG induced groups (0.25, 0.5 and 1 mM respectively). c Size exclusion chromatography profile (SEC). d SDS PAGE of a few SEC purified fractions (constant volume) obtained from peak 2 of c. e–g Characterization of IpaC. e SDS PAGE of expressed IpaC. Lanes represent bacterial cell lysates of uninduced control and IPTG induced groups (0.25, 0.5 and 1 mM respectively). f SEC profile (at 220 nm as Tryptophan is absent). g SDS PAGE of a few SEC purified fractions (constant volume) obtained from peak 2 of f. h and i Circular dichroism spectrogram of IpaB44-310 and IpaC, respectively. j and k Western blot of IpaB44-310 and IpaC respectively (same fraction obtained from three different batches separately loaded in the three lanes), with anti-mouse anti-histidine tagged primary antibody and goat-anti-mouse HRP-labelled secondary antibody