Fig. 2

hADMSCs-Exo inhibits hepatic stellate cell proliferation by impeding cell cycle progression and inducing apoptosis. A A schematic representation of the experimental design. B In vitro cell viabilities of activated LX-2 cells incubated with hADMSCs-Exo at the indicated concentration in the presence of TGF-β1 (10 ng/ml) for 24 h, 48 h or 72 h, (n = 5). C IC50 were analysed in activated LX-2 cells exposed to hADMSCs-Exo for 24 h. D Edu assay showed that HSCs proliferation was suppressed by hADMSCs-Exo in a concentration-dependent manner. EdU% is used as an approximation for proliferation rate. E Cell cycle analysis showed an increase in the sub-G1 subpopulation and cell cycle arrest after hADMSCs-Exo. F The quantitative analysis of the apoptosis was performed using the Annexin-FITC staining based flow cytometry. Exo, exosome. Data are presented as means with SEM (n = 3 independent experiments). ns, not significant, *p < 0.05, **p < 0.01 and ***p < 0.001