Fig. 3

hADMSCs-Exo internalized by LX2 cells exhibit antifibrotic and anti-EMT effect in vitro. A Representative phase-contrast images of quiescent HSCs and aHSCs were treated with hADMSCs-Exo at the indicated concentration in the presence of TGF-β1 (10 ng/ml). Analyses were conducted 24 h after the indicated treatments. Scale bars, 100 mm. B Representative immunofluorescence images of α-SMA (red) and DAPI (blue) of aHSCs were treated with hADMSCs-Exo. Scale bars, 100 mm. C Western blot analysis of LX-2 cell incubated with hADMSCs-Exo for 24 h in the presence of TGF-β1 (10 ng/ml). Data expressed as the mean ± SEM (n = 3 independent experiments). ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001