Fig. 6

Tumor explant supernatant induces functional remodeling of the macrophages. a Schematic overview of the experimental design. b Relative mRNA expression of Cxcl9, Cxcl10, iNos, IL-1β, and TNFα in bone marrow-derived macrophages (BMDMs) treated with tumor explant supernatant (TES) obtained from tumors of non-responders or responders for 24 h (n = 3/group). Data are presented as mean ± SEM. Statistical significance was determined using the two-tailed Student’s t-test. Data shown are representative of three independent experiments conducted in triplicate. *p < 0.05; **p < 0.01