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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling

Fig. 3

EIF4A3 promotes the biogenesis of circCABIN1. A Schematic image. Screening of RNA binding proteins by mass spectrometry. B The expression of circCABIN1 in LN229 cells was analyzed by qRT-PCR after indicated transfection. C The expression of circCABIN1 was analyzed by qRT-PCR after overexpress or knockdown EIF4A3. D Schematic image. Binding site of EIF4A3 and circCABIN1 pre-mRNA. E–F Pull-down silver staining and western blotting were used to demonstrate the interaction between EIF4A3 and the circCABIN1 pre-mRNA upstream and downstream region in LN229 cells. G RIP assay to verify EIF4A3 binding at the putative sites. H Schematic image. Construction of wild-type and deletion mutant expression plasmids for EIF4A3, and the expression of circCABIN1 in LN229 cells was analyzed by qRT-PCR after indicated transfection. I The expression of EIF4A3 was analyzed by western blot after overexpressed circCABIN1. J The expression of EIF4A3 was analyzed by western blot in TMZ resistant and parental cells. K The expression of EIF4A3 in relapsed or non-relapsed patients and representative EIF4A3 IHC images in GBM section of indicated groups. L Pearson correlation analysis of circCABIN1 and EIF4A3 (p = 0.0001). M The expression of EIF4A3 in different stages GBM and Kaplan–Meier analysis of OS in the high and low EIF4A3 groups in public database. N TMZ resistant cells administrated with shNC or shEIF4A3 were subjected to FACS to detect apoptosis. O The effect of shEIF4A3 and shEIF4A3 plus circCABIN1OE on TMZ resistant cells by CCK-8 assay. Results are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. NS, not significant

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