Fig. 2
From: Near-infrared light triggered in situ release of CO for enhanced therapy of glioblastoma
Construction of PDT combined with CO therapy platform. a UV–vis absorption spectra of UCNPs, UCNPs@Ce6, UCNPs@3HBQ and UCNPs@Ce6/3HBQ. b Fluorescence spectra of CO probe system (5 μM FL-CO + 5 μM PdCl2) incubated with UCNPs@Ce6/3HBQ and irradiated with 808-nm laser (0.3 W/cm2). c Fluorescence spectra of DCFH incubated with UCNPs@Ce6/3HBQ and irradiated with 808-nm laser (0.3 W/cm2). CLSM images of U87MG cells treated with (1) control, (2) UCNPs@3HBQ, (3) UCNPs@Ce6, (4) UCNPs@Ce6/3HBQ, (5) NIR, (6) UCNPs@3HBQ + NIR, (7) UCNPs@Ce6 + NIR and (8) UCNPs@Ce6/3HBQ + NIR after incubation with FL-CO + PdCl2 d or DCFH-DA f for 20 min. Nuclei were stained with Hoechst 33342. Scale bar: 20 µm. Average fluorescence intensity of FL-CO e and DCF g in U87MG cells treated with different conditions in d and f, respectively