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Fig. 8 | Journal of Nanobiotechnology

Fig. 8

From: Enhanced mucosal immune responses and reduced viral load in the respiratory tract of ferrets to intranasal lipid nanoparticle-based SARS-CoV-2 proteins and mRNA vaccines

Fig. 8

Detection of SARS-CoV-2 specific IgG antibody response in the BAL fluid of vaccinated and virus challenged ferrets. One-year-old male ferrets were vaccinated twice at 3 weeks interval intranasally with liposomes entrapped either mRNA or proteins of SARS-CoV-2 S-RBD and N along with monosodium urate crystal adjuvant and challenged with SARS-CoV-2 intranasally. The BAL fluid samples collected at DPC 7 and 14 were analyzed for S-RBD and N protein specific antibodies by ELISA. A–D S-RBD + N proteins-based vaccinates; E–H S-RBD + N mRNA-based vaccinates. Each marking is the mean of 3 animals ± SEM. Statistical analysis was performed by using Two-way ANOVA followed by Bonferroni test. Letter a—indicates the significance between SRBD + N mRNA + Adjuvant or SRBD + N proteins + Adjuvant VS SRBD + N mRNA + Adjuvant or SRBD + N proteins + Adjuvant in liposomes; b—significance between empty liposomes VS SRBD + N mRNA + Adjuvant or SRBD + N proteins + Adjuvant in liposomes at indicated dilutions

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