Fig. 2

CC700 resin enables consistent protein assembly purification across a range of pH and salt conditions. a Recombinant expression of γPFD in E. coli following inducement with IPTG as shown by SDS-PAGE. b TFF with a 1 MDa filter does not remove contaminant proteins from a bacterial lysate but is effective in removing nucleic acids. c UV chromatograms of MMC purifications showing comparable performance in a range of buffered solvents. Pre-processing with TFF increases resin binding capacity. d Comparison of an early fraction from MMC performed with different buffered solvents confirms that MMC performs comparably in all solvents except for ammonium sulfate. Omitting TFF pre-processing results in lesser purity. e Large protein assemblies purified using MMC can be further purified with a TX-114 phase separation procedure. f Transmission electron microscopy reveals contamination, possibly lipidic in nature, without TX-114 post-processing. LS: low salt (100 mM NaCl); NS: no salt; HS: high salt (1 M NaCl); AS: ammonium sulfate (1 M); TX-114: Triton X-114