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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates

Fig. 3

A strategy to attach and interspace functional proteins to γPFD filaments. a Predicted structural protein models of γPFD-SpyT and the functionalization subunits that are also represented as schematic icons. b Structural model of a γPFD-SpyT filament with SpyTag domains protruding from the C-termini of each subunit. c The SpyTag domains on γPFD-SpyT filaments can be blocked by the covalent attachment of SpyC subunits. d Attachment of CFP-SpyC and YFP-SpyC to the γPFD-SpyT filament via SpyTag/SpyCatcher covalent interactions. In this model, the CFP-SpyC and YFP-SpyC are randomly interspaced by SpyC. e Maximal density of CFP-SpyC and YFP-SpyC subunits attached to a γPFD-SpyT filament should be achieved by omitting the SpyC subunit

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Journal of Nanobiotechnology

ISSN: 1477-3155

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