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Table 2 Volumes of mixed lysates used for each functionalized filament

From: A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates

Lysate

Mix 1 (mL)

Mix 2 (mL)

Mix 3 (mL)

Mix 4 (mL)

Mix 5 (mL)

Mix 6 (mL)

Mix 7 (mL)

γPFD-SpyT

2

2

2

2

2

2

2

CFP-SpyC

0

0.25

0.5

0.75

1

1.25

1.5

YFP-SpyC

0

0.25

0.5

0.75

1

1.25

1.5

SpyC

3

2.5

2

1.5

1

0.5

0