From: A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates
Lysate
Mix 1 (mL)
Mix 2 (mL)
Mix 3 (mL)
Mix 4 (mL)
Mix 5 (mL)
Mix 6 (mL)
Mix 7 (mL)
γPFD-SpyT
2
CFP-SpyC
0
0.25
0.5
0.75
1
1.25
1.5
YFP-SpyC
SpyC
3
2.5