Fig. 6
Characterization of BSA-GOx-NPs. A, B Flow cytometry analysis of the uptake of 2-NBDG by eutopic stromal cells and ectopic stromal cells. All representative histograms A were quantified and mean fluorescence values ± SEM B are from three independent experiments. Eutopic stromal cells and ectopic stromal cells in each experiment were isolated and pooled from 3 to 4 mice. C, D. TEM image C and hydrodynamic diameters D of BSA-GOx-NPs. Scale bar is 200 nm. N = 3. E Zeta potential of BSA-NPs and BSA-GOx-NPs. N = 3 per group. F Glucose content-dependent H2O2 concentrations in “BSA-NPs + glucose” and “BSA-GOx-NPs + glucose” samples. The incubation time for the mixture of BSA-NPs or BSA-GOx-NPs and glucose was 1 h. N = 3 per group. G Cell viability curves for BSA-GOx-NPs and free GOx. N = 3. Data are shown as mean ± SEM and analyzed by two-tailed t-test. *P < 0.05