Fig. 7

Combined therapy reduced inflammation at the injury site via the Piezo1/NF-κB pathways. A WB was used to assess NF-κB/pNF-κB protein levels. B The expression of inflammation markers (CD86, iNOS, and TNF-α) at the lesion site was analysed by qPCR one week after injury. C Representative immunofluorescence staining images for TNF-α (green) seven days after surgery. Cell nuclei were stained with DAPI (blue). D Amplified images of each group. E Relative intensity of TNF-α in the lesion site