Scheme 1

Sequential scheme of the studies performed. MgFe-LDH/NT3 nanoparticles (NPs) were synthesized and then combined with ultrasound (US), and the effects of combined therapy were tested both in vitro and in vivo. In vitro, the roles of promoting neural differentiation and suppressing inflammation were investigated. In vivo, the therapeutic effects of combined therapy on SCI model mice were analyzed, further Piezo1 was identified via RNA-seq analysis and the underlying mechanisms were revealed then. The figure was created with BioRender.com