Fig. 9
From: Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization
Maleimide-activated SpyTag003-SpyCatcher003 functionalization of GVNPs. A mKate2 fluorescence (588/633 nm) of maleimide-activated SpyTag003- mKate2.SpyCatcher003, B Phase contrast and fluorescence images of the maleimide-activated SpyTag003- mKate2.SpyCatcher003 (bottom) and the GVNPs (top) (Image scale 38 \(\mu m\) × 38 \(\mu m\)), C Estimation of GVNPs bound esterase activity. TF: Top fraction, SF: subnatant fraction.