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Fig. 9 | Journal of Nanobiotechnology

Fig. 9

From: Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy

Fig. 9

The effect of (Lipo + M)@E NPs on the levels of PCSK9 and OPN in ApoE−/− atherosclerotic mice. Histochemistry photographs (A) and quantitative (B) of aortic root paraffin sections stained with anti-PCSK9. (C) Serum PCSK9 levels in ApoE−/− mics after different treatments were detected by Elisa. (D) Frozen sections of aortic root were prepared for double immunofluorescence staining. Green indicated PCSK9, red indicated α-SMA, and yellow indicated PCSK9-positive VSMCs in plaque. (F) Immunofluorescence staining using anti-OPN (green) and anti-α-SMA (red), yellow indicated OPN-positive VSMCs in plaque. (E&G) Quantification analysis of PCSK9 and OPN in VSMCs. 1: Control, 2: Model, 3: Evol, 4: Lipo@E NPs, 5:(Lipo + M)@E NPs. Scale bars = 500 µm. Data are means ± SD, n = 3, ##P < 0.01, ###P < 0.001 vs. the Control. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the Model

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