Fig. 2
Characterization of LPS-BMSCs. (A) The morphology, adipogenic and osteogenic differentiation of BMSCs(a-c) and LPS-BMSCs(d-f). Scale bar: 50 μm. (B) Flow cytometric analysis of the immunophenotype of BMSCs(a) and LPS-BMSCs(b). Blue filled lines indicate lgG controls, red filled lines the respective antibodies. (C) Apoptosis of BMSCs and LPS-BMSCs treated with H2O2 for 24 h assessed by Flow cytometry. (D) RAW264.7 stimulated with LPS (100 ng/mL) +IFN-γ (20 ng/mL) or IL4 (20 ng/mL) treated with or without LPS-BMSC membrane vesicles (BMSCm) for 24 h. Blue: DAPI; Green:CD86 (M1 marker); Red:CD206 (M2 marker). Scale bar: 50 μm.