Fig. 4

The anti-inflammatory effect of FZ/MER-AgMOF@Bm in vitro. (A) RAW264.7 were stimulated with LPS (100 ng/mL) + IFN-γ (20 ng/mL) for M1-like polarization and treated indicated reagents for 24 h. (B) RAW264.7 were stimulated with IL-4 (20 ng/mL) for M2-like polarization and treated with indicated reagents for 24 h. Blue: DAPI; Green:CD86 (M1marker); Red:CD206 (M2 marker). Scale bar: 50 μm. (C) ELISA for IL-6, IL-1β, TNF-α and IL-10 in the supernatants of indicated reagents-primed RAW 264.7 stimulated with LPS (1 µg/mL) for 24 h. Data are derived from three independent experiments and presented as mean ± SEM in the bar graphs. *P < 0.05, **P < 0.01, ***P < 0.001