Figure 6
Fluorescence expression of BCG. Tdtomato fluorescence protein expressed by bacillus Calmette-Guérin (BCG) allows fast detection of viable bacteria cells under treatment with NPs. (A) Correlation of the number of BCG and fluorescence level was determined by CFU, R2 = 0.994. At day zero, fluorescence of BCG was 701.7 ± 61.6. After five days incubation with NPs, (B) Fluorescence of BCG with 0.1, 1, 5, 10 μg/ml citrate Au NPs. (C) Fluorescence of BCG with 1, 5, 10, 20 μg/ml PAH Au NPs. (D) Fluorescence of BCG with 1, 5, 10, 20 μg/ml Ag NPs. The experiment was repeated three times and the results are shown in mean ± SD. Comparison was made with NP-free BCG control to determine the antibacterial effects. *p<0.05: significant difference from NP-free BCG control.