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Figure 5 | Journal of Nanobiotechnology

Figure 5

From: Chimeric Hepatitis B core antigen virus-like particles displaying the envelope domain III of dengue virus type 2

Figure 5

Competitive ELISA. Anti-EDIII mAb24A12 (panel A), anti-EDIII-T antiserum (panel B) and anti-HBcAg-EDIII-2 antiserum (panel C) were separately pre-incubated with varying concentrations of purified HBcAg (green curves) and HBcAg-EDIII-2 (red curves) VLPs, and analyzed for residual anti-EDIII-2 antibodies using purified P. pastoris-expressed EDIII-2 antigen as the coating antigen. ELISA reactivity in the absence of any added protein in the pre-incubation step was taken to represent 100%. The regression equations for the HBcAg-EDIII-2 competition assays (red curves) are shown just above the horizontal axis in each panel.

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