Figure 1

Immunostaining of intracellular EGFR in medulloblastoma. A. Labeling of EGFR using an antibody that recognizes an internal isotope, iEFGR (Alexa 488; green), and of the endocytic pathway using Transferrin (Tf) (purple) in medulloblastoma (MB) cells; B. Labeling of EGFR in MB using Qdots functionalized with biotinylated iEFGR (purple) and of the endocytic pathway using Tf (green); C. Percentage co-localization between Tf and iEGFR Ab, and Tf and Qdot:iEGFR Ab complexes. Nuclei are indicated by the letter "N". Scale bar equals 50 μm in both confocal images.