Figure 2

ELISPOT assays of mice immunized with different forms of nanoparticles. Mice, 5 per group, were immunized three times by the intraperitoneal route with 100 μg of recombinant PapMV NP-12, PapMV NP-C and PapMV nanoparticles or discs. Two weeks after the second boost, spleens were extracted and ELISPOT assays performed. The precursor frequency of specific T cells was determined by subtracting the background spots in media alone from the number of spots seen in wells reactivated with NP_147-155 peptide. *** p ≤ 0.001 compared to all groups.