Figure 5From: Elastomeric microparticles for acoustic mediated bioseparationsUsing NACPs to transport PACPs to the pressure antinode. Fluorescence images demonstrate the ability to use NACPs to transport PACPs to the pressure antinode within an acoustofluidic device. (A) As a negative control, PDMS microparticles (non-biotinylated, encapsulated with Nile Red fluorophore) were mixed with streptavidin polystyrene microparticles (green, 6 μm diameter). The lack of binding between the non-biotinylated PDMS and streptavidin polystyrene particles results in their transport to the antinode and node, respectively. (B) The high affinity between PDMS microparticles (biotinylated, encapsulated with rhodamine B fluorophore) and streptavidin polystyrene microparticles (green, 6 μm diameter) generate particle complexes that transport collectively to the pressure antinode within an ultrasound standing wave. Images acquired in the absence of flow with a 1:10 ratio of polystyrene:PDMS. Dashed lines are included to demarcate the channel boundaries. Scale bars represent 200 μm.Back to article page