Figure 3
Microfluidization processing effects on size characteristics of GLA-AF. (a) Effect of microfluidizing pressure and number of discrete passes on unbuffered GLA-AF particle size (0.1 mg/ml GLA target concentration). (b) Effect of microfluidizing pressure and number of discrete passes on unbuffered GLA-AF size polydispersity index. Two batches were manufactured at each pressure and monitored for size and PdI at 0, 2, 5, 10, 15, and 20 passes with the exception of one of the 10,000 psi batches where the 2nd pass measurement was missed so a 3rd batch was manufactured at 10,000 psi and measured at 0, 2, and 5 passes only.