Figure 7
(A) Internalozation of MC-IOPs in RAW cells. Cells were cultured with different volume of MC-IOPs (11.2 mg/ml). Cytospin slides were stained with Prussian blue (iron staining) for RAW cells; (a) control cells, (b) and (c) cells incubated with 10 and 20 μl MC-IOPs for 5 h. Inset figure indicate the higher magnification and black arrow denote cell label with particles. Scale bars represent 10 μm. (B) Iron content in RAW cells. Cells were cultured with different concentration of MC-IONPs for 2 h, and incubated for 24 h with fresh medium. C, 1, 2 and 3 represent 0, 5, 10 and 20 μl of MC-IOPs from the stock 11.2 mg/ml, respectively). (C) Flow cytometry of RAW cells incubated with different concentrations of MC-IOPs as described in (A). The SSC signal (SSC-H) is increased with increased concentrations of MC-IOPs. Quantitative iron assessment was performed with a colorimetric method. Values are means of ± S.D. of iron content per single RAW cells (pg).