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Table 1 Determination of serum CRP concentration using different RAP assay formats.

From: A high sensitivity assay for the inflammatory marker C-Reactive protein employing acoustic biosensing

 

Cardiovascular Risk (AHA/CDC guidelines)/[CRP] g/mL

 

LOW <1

Ave 1 – 3

HIGH 3 – 5

V. HIGH > 10

Spiked Serum [CRP]

0.1

1.2

3.61

10.4

34.8

94.8

hsCRP ELISA

0.092

1.26

4.35

9.6

44

116

Direct Capture

3.728

4.53

7.56

11.9

41

113

Homogenous Sandwich

3.4

3.4

6.3

15.5

53

120

Direct Sandwich

0.14

1.45

3.3

9.9

45.5

114

  1. Results are compared with that obtained by commercial hsCRP ELISA.
  2. Cardiovascular risk is considered at serum levels > 3 μg/ml. Normal horse serum was independently spiked with human CRP, these samples were diluted from 1/100 to 1/6000 for assay. Sandwich assays using RAP employ 0.225 μg/mL Sheep anti-CRP.
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Journal of Nanobiotechnology

ISSN: 1477-3155

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